Establishment of hsp90 overexpressing cell line and analysis of the binding ability of hsp90 with substrate proteins 90高表達(dá)細(xì)胞系的建立及其對底物蛋白結(jié)合能力的影響
The chemical structures of alginate and chitosan were analyzed , and a comparative study was conducted of the absorbability , metal ion binding ability and antimicrobial property of alginate and chitosan fibers 分析了海藻酸和甲殼胺的化學(xué)結(jié)構(gòu),對其吸附性能、結(jié)合重金屬離子的能力以及抗菌性能作了比較。
The result of water - binding ability test including water - retention ability , especially water - adsorption ability , showed great enhancement of hydrophilicity of the modified pdl - la scaffold , which would favor for the 3d cell culture 該改性技術(shù)可顯著提高聚乳酸支架的親水性,尤其可顯著提高聚乳酸支架的吸水能力,非常有利于三維支架的細(xì)胞培養(yǎng)。
How many what heart output blood measures , the size of lung respiration intensity , muscle is able - bodied degree , outside indicating a person is right , the reaction of all sorts of environments mixes the bound ability of meet an emergency , it is the mark of vitality 心臟輸出血液量的多少,肺臟呼吸強(qiáng)度的大小,肌肉強(qiáng)健程度等,標(biāo)志著一個人對外界各種環(huán)境的反應(yīng)和應(yīng)變能力,是生命力的標(biāo)志。
Rab7 mediates transport of proteins from early endosome to late endosome or fusion of early endosome with late endosome , while rab9 mediates the retransport of m6pr from late endosome to trans - golgi network . the gtp / gdp - binding ability and intrinsic gtpase activity of rab proteins make them exist in rab - gtp or rab - gdp status . the switching of rabs between gtp - bound ( active ) and gdp - bound ( inactive ) forms requires other regulatory proteins , including guanosine exchange factor ( gef ) , gdp dissociation inhibitor ( gdi ) and gtpase activating protein ( gap ) , and lays the foundations of rab function Rab蛋白家族成員和其他小g蛋白一樣可以結(jié)合gtp gdp ,在自身gtp酶的水解作用和其他蛋白( gef 、 gap 、 gdi )的調(diào)節(jié)作用下發(fā)生gtp (活化狀態(tài))和gdp (無活性)結(jié)合形式的轉(zhuǎn)變;同時rab蛋白也在膜結(jié)合性( membrane - bound )和胞漿可溶性( cytosolic )之間變化,一個變化過程對應(yīng)著一個功能循環(huán),接著rab蛋白回到初始狀態(tài)參與下一個循環(huán)。
To neutralize the function of cam , the cam binding domain peptide from bovine calcineurin a subunit was chosen because the pre - experiment demonstrated that bovine calcineurin could bind to calmodulin strongely in an acidic and high calcium concentration condition which is similar to the cell wall environment . in order to increase the binding ability of the chimeric secreted calmodulin binding peptide , the plasmid containing two copies of calmodulin binding domain were constructed 它共有26個氨基酸,具有信號肽的典型特征。為了削弱質(zhì)外體cam的功能,選擇了牛腦鈣調(diào)神經(jīng)磷酸酶can ( calcineurin )的a亞基的cam結(jié)合域。預(yù)實(shí)驗(yàn)結(jié)果表明,在近似于胞外環(huán)境的酸性高鈣條件下can與cam有強(qiáng)結(jié)合。
Results show that vp37 protein can bind single strand nucleic acid cooperatively and nonspecificallty , and the vp37 - ssrna complex was stable at high salt concentrations , suggesting vp37 is a possible mp . vp37 is the only protein characterized so far showing rna - binding ability in genus fabavirus 為了驗(yàn)證vp37是否具有核酸結(jié)合能力,我們利用6his - vp37蛋白進(jìn)行了核酸結(jié)合實(shí)驗(yàn),結(jié)果表明vp37是一種能非特異性結(jié)合單鏈核酸的蛋白;其在結(jié)合核酸時具有協(xié)同性; na ~ +的變化對其核酸結(jié)合能力影響較小。